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The antibody is from purified rabbit serum by affinity purification through sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of the gene has actually been observed in many tumors, which leads to chromosome instability, and therefore may perhaps add to tumorigenesis. This protein was uncovered to affiliate with, and be linked to, the phosphorylation of NPAT protein (nuclear protein mapped to your ATM locus), which participates in cell-cycle controlled histone gene expression and plays a crucial job in advertising mobile-cycle development during the absence of pRB. Two alternatively spliced transcript variants of the gene, which encode unique isoforms, have been explained. Two further splice variants were described but in depth nucleotide sequence information will not be but available.
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Blocking peptides are peptides that bind particularly for the focus on antibody and block antibody binding. These peptide ordinarily contains the epitope acknowledged from the antibody.
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This guide outlines further more doable optimization of cellular and immunoassay parameters to make sure the very best effects are...
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This information outlines even further doable optimization of mobile and immunoassay parameters to guarantee the very best effects are...
Antibodies sure to the blocking peptide no more bind to your epitope to the focus on protein. By comparing the staining from your blocked antibody compared to the antibody alone, one can see which staining is unique.
Concentrate on Facts Cyclin E belongs to your hugely conserved cyclin loved ones, whose associates are characterized by a extraordinary periodicity in protein abundance from the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit unique expression and degradation patterns which add into the temporal coordination of every mitotic occasion. Cyclin E forms a fancy with and functions being a regulatory subunit of CDK2, whose activity is necessary for cell cycle G1/S transition. This protein accumulates at the G1-S period boundary and it is degraded as cells development by way of S phase.
The measurement of protein phosphorylation is a useful gizmo for measuring the modulation of receptor activation by equally antibodies...
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